Ekspresi gen penyandi ACCase subunit biotin karboksilase dari mesokarp buah kelapa sawit pada Escherichia coli Expression of gene encoding ACCase subunit biotin carboxylase from oil palm fruit mesocarp in Escherichia coli
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چکیده
منابع مشابه
Cloning and evaluation of gene expression and purification of gene encoding recombinant protein containing binding subunit of coli surface antigens CS1 and CS2 from Enterotoxigenic Escherichia coli
Background & Objective: Enterotoxigenic Escherichia coli (ETEC) is a major causative agent of diarrhea. Enterotoxins and the colonization factors (CFs) are major virulence factors in ETEC infections. The bacterium binds to the intestinal epithelial cell surface through colonization factors and produces enterotoxins that cause excessive fluid and electrolyte secretion in the lumen of the intesti...
متن کاملThe biotin carboxylase-biotin carboxyl carrier protein complex of Escherichia coli acetyl-CoA carboxylase.
Escherichia coli acetyl-CoA carboxylase (ACC) is composed of four different protein molecules. These proteins form a large but very unstable complex. Hints of a sub-complex between the biotin carboxylase (BC) and biotin carboxyl carrier protein (BCCP) subunits have been reported in the literature, but the complex was not isolated and thus the protein stoichiometry could not be determined. We re...
متن کاملBiochemical and molecular biological characterization of CAC2, the Arabidopsis thaliana gene coding for the biotin carboxylase subunit of the plastidic acetyl-coenzyme A carboxylase.
The biotin carboxylase subunit of the heteromeric chloroplastic acetyl-coenzyme A carboxylase (ACCase) of Arabidopsis thaliana is coded by a single gene (CAC2), which is interrupted by 15 introns. The cDNA encodes a deduced protein of 537 amino acids with an apparent N-terminal chloroplast-targeting transit peptide. Antibodies generated to a glutathione S-transferase-CAC2 fusion protein react s...
متن کاملPurification and characterization of intact and truncated forms of the Escherichia coli biotin carboxyl carrier subunit of acetyl-CoA carboxylase.
Biotin biosynthesis and retention in Escherichia coli is regulated by the multifunctional protein, BirA. The protein acts as both the transcriptional repressor of the biotin biosynthetic operon and as a ligase for covalent attachment of biotin to a unique lysine residue of the acetyl-CoA carboxylase. Biotinyl-5'-AMP is the activated intermediate for the ligase reaction and the allosteric effect...
متن کاملcloning and expression of beta subunit gene of phycocyanin from spirulina platensis in escherichia coli
conclusions over-expression of the synthetic cpc/β protein in the bacterial system (escherichia coli bl-21) showed that e. coli can be used as a basis for further research to produce this desired protein in large quantities. results the sds-page analysis and dot blotting confirmed the production of recombinant c-pc/β in the bacterial expression system. over-expression of cpcb gene was optimized...
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ژورنال
عنوان ژورنال: E-Journal Menara Perkebunan
سال: 2016
ISSN: 1858-3768,0125-9318
DOI: 10.22302/iribb.jur.mp.v82i1.29